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2-10 nmol of L-glutamate standards and the cell lysate were mixed with reaction buffer (final concentrations of 36.8 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (pH 7.1), 2.1910-5 U L-glutamate oxidase, 1 U peroxidase, 0.8 mM 3.5-dimethoxy-N-ethyl-N-(2-hydroxy-3-sulphopropyl) aniline (sodium salt) (DAOS), 0.8 mM aminoantipyrine)

Yeast (candida) can invade the lining of the intestinal wall to cause SIFO

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Peptides perform best when they appear relatively high up--though exact placement isn't a perfect indicator because of labeling regulations

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